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Power of Mediso's nanoScan combined systems: Perfect Co-Registration

by in Molecular Imaging

In the first published article from MSKCC (Carney, B. et al. Non-invasive PET Imaging of PARP1 Expression in Glioblastoma Models. Mol Imaging Biol 1–7 (2015)), using the nanoScan PET/MRI (1T) small animal imaging system, in vivo whole body PET/MRI imaging of [18F]PARPi in orthotopic brain tumor-bearing mice is referenced.

[18F]PARPi is a selective PARP1 imaging agent that can be used to visualize glioblastoma in xenograft and orthotopic mouse models with high precision and good signal/noise ratios offering new opportunities to non-invasively image tumor growth and monitor interventions.

Figure 6 in the article shows coronal views of contrast-enhanced MRI, [18F]PARPi PET images, and fused PET/MRI of orthotopic U251 MG tumor-bearing mice. In the top row the mouse receivied only [18F]PARPi, in the bottom row the mouse receivied [18F]PARPi after a 500-fold excess of olaparib.

Non-invasive PET/MRI Imaging of PARP1 Expression in Glioblastoma Models with nanoScan PET/MRI (1T)

The animals were injected with 200 µCi of [18F]-PARPi and a 20 minutes static PET scan was acquired 2 hours post injection. 200 µL of diluted gadopentate dimegumine in saline solution  was administered intravenously one minute prior to MRI acquisition. Tumor regions were identified on anatomic images acquired using a post-contrast T-weighted spin-echo (SE) acquisition. The co-localization of [18F]PARPi and tumor in PET/MRI studies was confirmed by ex vivo autoradiography. In PET/MRI fusion images, accumulation in the tumor was co-aligned with the orthotopic tumor on MRI. In mice receiving an injection of olaparib ahead of the radiotracer, the [18F]PARPi tumor uptake was negligible.

It's important to note that no further or manual co-registration was required at all as the PET/MRI studies performed on the nanoSCan PET/MRI are co-registered by nature due to the common gantry and automated acquisition system. The very same images are displayed in the viewer when the dual-modality study is loaded from the DICOM server after reconstruction. This gives scientists confidence when evaluating multi-modal data; changing animal physiology and data obtained at different times won't distort the findings.

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Illes Muller is the Managing Partner of Mediso USA. After completing the Master in Engineering-Physics and Master in Biomedical Engineering degrees from the Budapest University of Technology and Economics, he gathered extensive experience in in vivo tomography system developments while working for Mediso Medical Imaging Systems in both clinical and preclinical projects. Including 4 years working as the Deputy Director of Development at 77 Elektronika, a Hungarian private company developing and manufacturing in vitro diagnostic medical devices, Mr. Muller has 10 years of experience in managing medical device development. As an active member of the imaging community, Mr. Muller a member of the World Molecular Imaging Society Industry Board.

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